Coding
TvoVMA-N

Part:BBa_K3308071:Design

Designed by: Jemy Varghese, Ripal Sheth, Victor So, Harrison Green, Melissa Marciesky   Group: iGEM19_Pittsburgh   (2019-10-06)


[Tvo VMA C48 N] + stop


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 18
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Design of this part was based on predicted best site to splice. The splice site was chosen according to biochemical requirements for interactions between either ends of intein.



Source

The source of the intein sequence and characterization data is InBase


References